Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows
نویسندگان
چکیده
منابع مشابه
Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species.
The rapid and reliable identification of clinically significant Mycobacterium species is a challenge for diagnostic laboratories. This study evaluates a unique sequence-dependent identification algorithm called MycoAlign for the differential identification of Mycobacterium species. The MycoAlign system uses pan-Mycobacterium-specific primer amplification in combination with a customized databas...
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The aim of this study was to determine the effect of yeast culture (Saccharomyces cerevisiae SC 47) addition in the diet of dairy cows on their rumen fermentation and milk production. Animals received a diet TMR based on good maize silage with a higher dry matter content (14 kg), 14 kg of lucerne-grass haylage, 5 kg of crushed ears of maize, 5 kg of beet pulp silage, 3 kg of crimped wheat, 2 kg...
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Feeding dairy cows for maximum milk production increases the risk of ruminal acidosis. Diets fed to high producing dairy cows in North America are typically highly digestible in the rumen and often lack sufficient physical fiber. Rapid fermentation of carbohydrates causes an accumulation of volatile fatty acids (VFA) in the rumen, leading to a drop in ruminal pH. Lack of sufficient physical fib...
متن کاملNucleotide sequence analysis of the Second Internal Transcribed Spacer (ITS2) in Hyalomma anatolicum anatolicum in Iran
Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...
متن کاملMultiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.
Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed spacer 1 (ITS1) and ITS2 regions and the 5.8S ribosomal DNA (rDNA) region of the fungi were amplified by using universal primers ITS1 and ITS4. The ITS2 region was simultaneously amplified by using uni...
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ژورنال
عنوان ژورنال: Animal
سال: 2016
ISSN: 1751-7311
DOI: 10.1017/s1751731116000768